E8781
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 | Immunoblotting Whole cell extract of human HEK-293T was separated on SDS-PAGE and blotted with Rabbit Anti-EDEM3 (C-terminal) (Cat. No. E8781). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 1:2,000 2. Antibody 1:2000 + EDEM3 immunizing peptide (human, 869-882) |
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E8781
Sigma
Anti-EDEM3 (C-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | Anti-ER degradation enhancer, mannosidase alpha-like 3 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 869-882 of human EDEM3, conjugated to KLH via an N-trerminal cysteine residue. The corresponding sequence is identical in mouse and rat EDEM3. |
| Physical form | Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~120 kDa |
| species reactivity | mouse, rat, human |
| application(s) | immunoblotting: 1:1,000-1:2,000 using whole extract of human HEK-293T cells |
| immunoblotting: 1:500-1:1,000 using whole extrct of rat PC12 cells | |
| immunoprecipitation: 1-2 μL using mouse 3T3 cell lysates | |
| Gene Information | human ... EDEM3(80267) |
| mouse ... Edem3(66967) | |
| shipped in | dry ice |
| storage temp. | −20°C |
